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THE EFFECT OF CHLORHEXIDINE DIHYDROCHLORIDE AND SODIUM FLUORIDE ON STREPTOCOCCUS

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Abstract

Chlorhexidine dihydrochloride¿Í ºÒÈ­³ªÆ®·ýÀº ±¸°­ ³» ¼¼±ÕÀ» ¾ïÁ¦ÇÏ°í Ä¡ÅÂÀÇ Çü¼ºÀ» ¾ïÁ¦Çϱâ À§ÇÏ¿© ¸¹ÀÌ »ç¿ëµÇ°í ÀÖ´Â ¹°ÁúÀÌ´Ù. ±×·¯³ª ±¸°­¿¡´Â ¿©·¯ Á¾·ùÀÇ ¼¼±ÕµéÀÌ »óÁÖÇϸç ÀÌµé ¹°Áú¿¡ ´ëÇÑ °¨¼ö¼ºµµ ´Ù¸£´Ù. º» ¿¬±¸¿¡¼­´Â chlorhexidine dihydrochloride¿Í ºÒÈ­³ªÆ®·ý¿¡ ÀÇÇØ Streptococcus mutansÀÇ ÀΰøÄ¡Å Çü¼ºÀÌ ¾ïÁ¦µÇ¾úÀ» ¶§ Streptococcus mutans, Streptococcus sobrinus, Streptococcus oralis, Streptococcus salivariusÀÇ Áõ½Ä¿¡ ¹ÌÄ¡´Â ÀÌµé ¹°ÁúÀÇ ¿µÇâÀ» °üÂûÇÏ¿© ´ÙÀ½°ú °°Àº °á°ú¸¦ ¾ò¾ú´Ù.
Streptococcus mutans¸¦ 8½Ã°£ ¹è¾çÇÏ¸é ¿ÍÀ̾ Çü¼ºµÈ ÀΰøÄ¡Å ¹«°Ô´Â 106.1¡¾18.1mgÀ̾úÀ¸³ª. 1.0¥ìM chlorhexidine dihydrochloride¸¦ ÷°¡Çϸé 5.1¡¾1.5mgÀ¸·Î °¨¼ÒµÇ¾ú´Ù(p<0.05). ÀÌ ¶§ ¹è¾ç¾×ÀÇ Èí±¤µµµµ °¨¼ÒµÇ¾ú´Ù. Streptococcus sobrinusÀÇ ¹è¾ç¾× Èí±¤µµ´Â ¾à°£ °¨¼ÒµÇ¾úÀ¸³ª Streptococus oralis¿¡¼­´Â °¨¼ÒµÇÁö ¾Ê¾Ò°í Streptococcus salivarius¿¡¼­´Â ¹è¾ç 8½Ã°£¿¡ °¨¼ÒµÇ¾ú´Ù°¡ ¹è¾ç 24½Ã°£¿¡ ´ëÁ¶±º°ú Â÷ÀÌ°¡ ¾ø¾ú´Ù. ¹èÁö¿¡ 3.0mM ºÒÈ­³ªÆ®·ýÀ» ÷°¡ÇÏ¿© Streptococcus mutans¸¦ 8½Ã°£ ¹è¾çÇÏ¸é ¿ÍÀ̾ Çü¼ºµÈ ÀΰøÄ¡Å ¹«°Ô´Â 267¡¾8.3mgÀ¸·Î °¨¼ÒµÇ¾ú´Ù(p<0.05). ÀÌ ¶§ ¹è¾ç¾×ÀÇ Èí±¤µµµµ °¨¼ÒµÇ¾ú´Ù. Streptococcus sobrinus¿Í Streptococcus oralis¿¡¼­ÀÇ ¹è¾ç¾×ÀÇ Èí±¤µµµµ °¨¼ÒµÇ¾úÀ¸³ª Streptococcus salivarius¿¡¼­´Â °ÅÀÇ °¨¼ÒµÇÁö ¾Ê¾Ò´Ù.
ÀÌ»óÀÇ °á°ú´Â Streptococcus mutansÀÇ ÀΰøÄ¡Å Çü¼ºÀ» ¾ïÁ¦ÇÏ´Â chlorhexidine dihydrochloride¿Í ºÒÈ­³ªÆ®·ý ³óµµ¿¡¼­ StreptococcusÀÇ ¿©·¯ Á¾ÀÇ °¨¼ö¼ºÀÌ °¢°¢ ´Ù¸£´Ù´Â °ÍÀ» ½Ã»çÇÏ¿´´Ù.
Chlorhexidine dihydrochloride and sodium fluoride have been used as agents inhibiting the replication of oral bacteria and the formation of dental plaque. There are various kinds of bacteria with different sensitivity against these agents. In this study, chlorhexidine dihydrochloride and sodium fluoride were studied about their effects
on the replication of Streptococcus mutans, Streptococcus sobrinus, Streptococcus oralis and Streptococcus sali¡þvarius at their concentrations inhibiting the formation of artificial plaque by Streptococcus mutans.
When Streptococcus mutans was cultured for 8 hours in the media added with 1.0tM chlorhexidine dihy¡þdrochloride, the weight of formed artificial plaque was decreased to 5.1¡¾1.5mg compared with 106.1¡¾18.1mg of the control (p(0.05). At the same time, the optical density of cultured media was decreased. The optical density of cultured media was slightly decreased in Streptococcus sobrinus, but was not decreased in Streptococcus oralis. The optical density of Streptococcus salivarius was decreased at 8 hours-incubation, was not decreased at 24 hours-incubation. When Streptococcus mutans was cultured for 8 hours in the media added with 3.0 mM sodium fluoride, the weight of formed artificial plaque was decreased to 26.7¡¾8.3 mg(p(0.05). At the same time, the optical density of cultured media was decreased. The optical density of cultured media was decreased in Streptococcus sobrinus and Streptococcus oralis, but was slightly decreased in Streptococcus salivarius.
These results suggest that at the concentration of chlorhexidine dihydrochloride and sodium fluoridehe inhibit¡þing the formation of artificial plaque by Streptococcus mutans, different species of genus Streptococcus show the different sensitivity against these agents.

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Streptococcus;Chlorhexidine dihydrochloride;ºÒÈ­³ªÆ®·ý;Streptococcus;Chlorhexidine dihydrochloride;Sodium fluoride

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